Valid for: 2024/25
Faculty: Faculty of Engineering LTH
Decided by: PLED B/K
Date of Decision: 2024-04-17
Effective: 2024-05-08
Main field: Biotechnology
Depth of study relative to the degree requirements: Second cycle, in-depth level of the course cannot be classified
Elective mandatory for: MLIV1
Elective for: B4-mb, B4-pt, MBIO1
Language of instruction: The course will be given in English on demand
The aim of the course is to give advanced knowledge about the technical use of enzymes and the possibilities to change and improve enzyme performance for adaptation to technical applications
Knowledge and understanding
For a passing grade the student must
Competences and skills
For a passing grade the student must
Judgement and approach
For a passing grade the student must
The theoretical part of the course describes how the catalysts of the biological world, the enzymes, can be used in a variety of contexts. Examples are the use in bioanalysis, refining of food, washing powder, textile treatment and transformation of chemicals.
The general properties of enzymes are well known from previous courses. In this course is described how enzyme molecules can be modified in order to get improved properties for technical use. Examples include genetic and chemical modification and immobilization. Immobilization, i.e. the coupling of enzymes to carrier material, is treated thoroughly. Likewise are the kinetic properties of immobilized enzymes and ways to quantify these properties emphasized.
In the cell the enzymes function in an aqueous environment. Under certain circumstances enzymes can also work efficiently in a non-aqueous environment or in an environment with a low water activity. This opens up for unique possibilities for an enzymatic synthesis of a number of substances, including chiral substances. This kind of use of enzymes in non-conventional media is treated in great detail.
The laboratory practical has two parts. One part where basic techniques concerning immobilization of enzymes are investigated and one research related part where untested experiments with enzymes are designed and carried out.
Grading scale: TH - (U, 3, 4, 5) - (Fail, Three, Four, Five)
Assessment: Written examination and reports from the laboratory practicals and literature discussion.
The examiner, in consultation with Disability Support Services, may deviate from the regular form of examination in order to provide a permanently disabled student with a form of examination equivalent to that of a student without a disability.
Modules
Code: 0117. Name: Enzyme Technology, Theory.
Credits: 4.5. Grading scale: TH - (U, 3, 4, 5).
Assessment: Written examination and passed literature discussion.
The module includes: The course descibes how the catalysts of the biological world, the enzymes, can be used in a variety of contexts. Examples are the use in bioanalysis, refining of food, washing powder, textile treatment and transformation of chemicals. The general properties of enzymes are well known from previous courses. In this course is described how enzyme molecules can be modified in order to get improved properties for technical use. Examples include genetic and chemical modification and immobilization. Immobilization, i.e. the coupling of enzymes to carrier material, is treated thoroughly. Likewise are the kinetic properties of immobilized enzymes and ways to quantify these properties emphasized. In the cell the enzymes function in an aqueous environment. Under certain circumstances enzymes can also work efficiently in a non-aqueous environment or in an environment with a low water activity. This opens up for unique possibilities for an enzymatic synthesis of a number of substances, including chiral substances. This kind of use of enzymes in non-converntional media is treated in great detail.
Code: 0217. Name: Enzyme Technology, Practicals.
Credits: 3.0. Grading scale: UG - (U, G).
Assessment: Written reports.
The module includes: The laboratory practical has two parts. One part where basic techniques concerning immobilization of enzymes are investigated and one reasearch related part where untested experiments with enzymes in organic solvents are designed and carried out.
Admission requirements:
The number of participants is limited to: 48
Course coordinator: Carl Grey,
carl.grey@biotek.lu.se
Course coordinator: Dr Cedric Dicko,
cedric.dicko@tbiokem.lth.se
Course homepage: http://www.tbiokem.lth.se/english/education/enzyme-technology/